Is it possible to watch the biological molecular clock ticking?
Yes, in fact it is! By tagging a so-called glock gene or a clock gene protein to a visible marker, one can watch the gene or protein activity in real time.

Video about Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus. Language: english.

The procedure of preparing slices containing the adult mouse hypothalamic suprachiasmatic nucleus (SCN), and a rapid way to culture the SCN tissue in organotypic culture condition, are reported. Further, the measurement of oscillatory clock gene protein expression using dynamic luciferase reporter technology is described.

PER2::LUC protein oscillations in individual neurons

Image

Real time recording of Period1 expression:

Per1-driven luciferase activity in rat SCN slice (Yamazaki et al 2000)

SCN explant cultures from transgenic animals

gabriella_lundkvist_work_410px.jpg

About me

I am a neuroscientist specialized in research on circadian rhythms, or so called "chronobiology", which is a research field that describes how daily fluctuations in sleep-wakefulness, social behavior, hormonal release, immune system mechanims and other bodily functions are controlled by a "body clock".

Curriculum vitae

  • Feb 2015
    Scientific coordinator, Max Planck Institute for Biology of Ageing, Cologne, Germany
  • Aug 2014
    Associate professor (habilitation), Department of Neuroscience, Karolinska Institutet, Stockholm
  • pubfacts
  • research gate
Copyright © 2018 www.gabriella-lundkvist.com All rights reserved. Contact | Login